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Induction and measurement of murine pulmonary fibrosis Pulmonary fibrosis was induced in female C57BL/6 mice (1012 weeks old) by a single intratracheal instillation of 50 l of bleomycin (3 U/kg, Sigma Aldrich, Taufkirchen, Germany) dissolved in sterile saline, and applied using the MicroSprayer Aerosolizer, Model IA-1 C (Penn-Century, Wyndmoor, PA)