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Both the Tet-inducible vector pRMC2 (Addgene, plasmid 68940) 70 and the mKate insert were digested with KpnI-HF and EcoRI-HF (New England Biolabs), gel purified using a QIAquick PCR purification kit (Qiagen), and ligated using T4 DNA ligase (New England Biolabs) at 16 C for 16 h

For men, andropause-related testosterone decline compounds the GH axis deterioration

Mech Ageing Dev 126(3):389398 Cascalheira JF, Gonalves M, Barroso M, Castro R, Palmeira M, Serpa A, Dias-Cabral AC, Domingues FC, Almeida S (2015) Association of the transcobalamin II gene 776CG polymorphism with Alzheimers type dementia: dependence on the 5, 10-methylenetetrahydrofolate reductase 1298AC polymorphism genotype

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