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C., Nguyen Dinh Cat, A., Rios, F

A two-step polymerase chain reaction (PCR) workflow was conducted for library preparation in accordance with procedures described in the Illumina 16S sample preparation guide

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In brief, sample fixation was achieved by 10% neutral-buffered formalin, followed by dehydration in serial grades of ethanol, then clearance with xylene and embedment into Paraffin/wax blocks

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