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FFA2 was knocked out by homologous recombination which substitutes 55 bp of FFA2 exon 1 with the -gal-neo cassette, shifting the downstream amino-acid sequence out of the reading frame

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"Today's announcement represents our single largest US manufacturing investment outside our home state of Indiana and will add to our ability to expand capacity to make both our existing and future pipeline of medicines right here in the Midwest," said Edgardo Hernandez, executive vice president and president of Lilly Manufacturing Operations