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Cells were washed three times with Krebs buffer (20 mM HEPES, 5 mM KH 2 PO 4 , 1 mM MgSO 4 , 1 mM CaCl 2 , 136 mM NaCl, 4.7 mM KCl, pH 7.4), then incubated with 500 g/ml 2-NBDG, a fluorescence-labeled deoxyglucose analog, for 20 min at room temperature

Its protective effects are primarily mediated through antioxidant and anti-inflammatory properties, as well as by modulating pathways such as RIP1/RIP3/MLKL, AKT/mTOR, and miR-22-3p/DPP4

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Although several in vivo and ex vivo studies have shown that NAC plays important biological actions that can potentially support its putative therapeutic roles, its effectiveness in clinical studies in addressing different pathological conditions still has conflicting results [4,9]

However, the results were not positive for cardiovascular death (RR 0.93