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Cells were incubated at 37 C for up to 5 minutes before aspirating the dissociation solution followed by addition of wash medium (N2B27) supplemented with 10 M ROCK inhibitor, 33 g /mL DNase I (Worthington), and 30 M actinomycin D

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AOD9604 did not, however, increase blood sugar levels or decrease insulin synthesis, in contrast to human growth hormone [2]

The question was whether transitioning them to orforglipron would preserve those results or whether they would regain the weight