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Temperature excursions above 8C denature the peptide structure irreversibly, eliminating therapeutic activity

ECG Recording ECGs were recorded continuously in deeply anesthetized rats for all three main leads, by positioning stainless steel electrodes on all four limbs using an ECG monitor with a 2090 programmer (Medtronic, Minneapolis, MN, USA) connected to a Waverunner LT342 digital oscilloscope (LeCroy, Chestnut Ridge, NY, USA) at 15 min, 24 h or 48 h ligation-time

Using intravenous vitamin B12 shows how different treatments are used around the world

The research was funded by the Koch Institute Dana-Farber Bridge Project and the National Institutes of Health.

Store peptides in non-cycling freezers or in the coldest, most stable location within frost-free units