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Crypt-laden Matrigel was dispensed onto 48-well culture plates, and following solidification of Matrigel droplets, the crypts were overlaid with the following medium: Advanced Dulbeccos Modified Eagles Medium/F12 (Thermon Fisher Scientific) supplemented with penicillin/streptomycin, 10 mM HEPES, 2 mM GlutaMAX, 1 B-27 Supplement (Thermo Fisher Scientific), 10 nM gastrin I (Sigma-Aldrich), 1 mM N-acetylcysteine (Sigma-Aldrich), and the niche factors of 100 ng/mL recombinant mouse Noggin (PeproTech), 50 ng/mL recombinant mouse EGF (Invitrogen), 10% R-spondin1 conditioned medium, 500 nM A83-01 (Tocris), 100 ng/mL human Wnt-3 (R&D system)

In the trial, 6.5% of participants taking Wegovy had a major adverse cardiovascular event compared to 8% on placebo, according to the FDA

Monique Ramsey (20:11): Exactly

References Mitchell JL, Lyons HS, Walker JK, Yiangou A, Grech O, Alimajstorovic Z, et al

Glucagon-like peptide-1 protects the murine hippocampus against stressors via akt and ERK1/2 signaling