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Research published in Aging shows that male late-onset hypogonadism, an age-related decline in testosterone secretion, correlates with dysfunction and accumulation of senescent Leydig cells in testes

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To determine the Fe 2+ content, 80 L of the supernatant was mixed with 80 L of the chromogenic solution and incubated at 37C for 10 min, and detected by a multifunctional electronic enzyme marker