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The columns were washed with 15 column volumes of buffer 1 followed by the elution with 200 mM imidazole, 50 mM Na x H y PO 4 , 300 mM NaCl, pH 8.0 at 4 C

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304, E113 (2013)

The drug could significantly prevent muscle atrophy among older adults, according to one clinical trial by BioAge in December involving 227 people age 65 or older