BSO is an inhibitor of GSH synthase that has been used to deplete GSH in vitro , including in macrophages ( We analyzed the gene expression profile and identified patterns of LPS-induced genes that were inhibited by endogenous GSH or that, on the contrary, required GSH for their induction
Yield: 800 mg, 90%
Astaxanthin has been studied for its role in supporting muscle endurance and reducing the time needed for recovery
Initial studies of several independent lines of poplar overexpressing -ECS showed no phenotypic effects (Noctor et al., 1998a)
The blots were incubated with primary antibodies against PGC-1 (Chemicon International, Temecula, CA) and AMP-activated protein kinase (AMPK) (Cell Signaling Technology, Beverly, MA), and the reaction products were visualized using a horseradish peroxidase-conjugated secondary antibody (Invitrogen, Carlsbad, CA) and enhanced chemiluminescence (Chemi-Lumi One Super, Nakarai Tesque, Kyoto, Japan) (Additional file 1: Figure S1)