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Avoid repeated freeze-thaw cyclesaliquot if necessary

5.3 Microbiota-targeted interventions Gut microbiota dysbiosis has been recognized as a central pathological mechanism in EDC

Neurochem Res 29 , 19231930 (2004)

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We then investigated the intracellular content of GSH and GSSG through a luminescence assay and we observed that the levels of GSH decreased in all cell lines in the passage from 2D to 3D grown condition, while the GSSG content showed an opposite trend (Fig