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The analysis shows that primitive protozoan parasites completely lack the TS/glutathione pathway

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Incubation of samples ( n = 3) was 1 hour at room temperature, after which the click reaction to DADPS biotin azide (Click Chemistry Tools) was performed by the addition of 1.88 l DADPS biotin azide, 25 l of THPTA:CuSO 4 100 mM: 20 mM, and 20 l of freshly dissolved 200 mM sodium ascorbate