POPs are mainly excreted through conjugation with GSH and this mechanism is extremely important for the health status, since exposure to POPs has been associated to diabetes, cardiovascular diseases and many other chronic diseases ( ROS production can occur at several intracellular sites but, for most cells, takes place in the mitochondria and the mitochondrial electron transport chain is the main cellular process of ROS generation in physiological circumstances ( 2 O 2 )
After 2 min, 0.1 ml samples were diluted to a final volume of 1 ml containing 1 mM GSH, 1 mM CDNB in 0.1 M potassium phosphate buffer, pH 6.5, according to the standard procedure of Habig et al
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This concentration is great for studies requiring larger doses, as it keeps the injection volume small