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This condition, known as functional hypothalamic amenorrhoea, is primarily related to low energy availability rather than simply low body weight or BMI alone

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After centrifugation for 10 min at 20,000 g and 4 C, supernatants were subjected for HPLC analysis using a DIONEX UltiMate 3000 system with Corona Veo SD Charged-Aerosol-Detector (Thermo Scientific GmbH, Dreieich, Germany)