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A quick way to decide: start with the solvent on the certificate of analysis, fall back to bacteriostatic water for a standard sequence, and only reach for acid or base when a peptide visibly resists water

Bullock BP, Heller RS, and Habener JF

Most courses last between 4 to 8 weeks

Some patients report ongoing symptoms such as persistent nausea, vomiting, abdominal pain, and delayed gastric emptying that do not resolve after discontinuation