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This review integrates mechanistic insights with translational advances, outlining how dysregulation of chaperones, autophagy-mitophagy, the ubiquitinproteasome system, and ER stress pathways drive human diseases, while highlighting emerging therapeutic platforms, from pharmacological chaperones and autophagy modulators to targeted protein degradation technologies, CRISPR screens, spatial biology, and AI-guided drug discovery

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The animals were immobilized with a dart gun 39 and a sample of blood was drawn from the jugular vein into EDTA-tubes (S-Monovette K3E, Sarstedt, Hildesheim, Germany) approximately 1530 minutes after darting

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