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The calibration curve was prepared using an analytical standard of curcumin (75% purity, Sigma-Aldrich, USA)

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The largest and most consistent pulse occurs during slow-wave sleep (stages 3 and 4), typically 60120 minutes after sleep onset

This evaluation was performed through an assessment of the expression of different surface molecules such as: CD13, CD29, CD44, CD73, CD90, CD105, and markers CD14, CD34, and CD45 [38]