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Each sample (8 l) was injected onto an Agilent Poroshell 120 EC-C18 (100 2.1 mm, 2.7 um) in a Shimadzu Nexera UHPLC system at a flowrate of 0.2 ml/min using a gradient elution created between buffer A (10 mM tributylamine aqueous solution adjusted pH to 4.95 with 15 mM acetic acid) and buffer B of methanol, from 10% to 40% (buffer B) in 30 min at room temperature
Zinman B, Wanner C, Lachin JM, etal
Key Dosage Guidelines Start with 250 to 500 mg daily if you are new to glutathione Increase to 500 to 1,000 mg per day based on your goals Two smaller doses spread across the day may be absorbed better than one large dose Always follow the recommended dosage on your supplement label Consult a healthcare professional before exceeding 1,000 mg per day When Is the Best Time to Take Glutathione Tablets
