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The standards and samples were then diluted 1:4 in assay buffer (I-Block buffer + 5% BSA)

A comparison of airway and serum matrix metalloproteinase-9 activity among normal subjects, asthmatic patients, and patients with asthmatic mucus hypersecretion

Similarly, trypanosomes utilize a highly specialized peroxisome, labeled as glycosome, for important metabolic functions, including glycolytic pathway, pentose-phosphate pathway, -oxidation of fatty acids, purine salvage, and biosynthetic pathways for pyrimidines, ether-lipids and squalenes (Haanstra et al., 2016)

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This piece gets into the science behind both methods