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In particular, TMAO is accumulated in one half the concentration of urea in shallow-sea elasmobranchs to counteract the deleterious effects of urea on cellular proteins (Seibel and Walsh, 2002)

3 Results 3.1 Bioanalytical analysis method development and validation We identified the protonated precursors ions for 7,8-DHF and D4-genistein (ISTD) at m/z 255 and 275, respectively