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Ischemia-Reperfusion Protocol A separate group of hearts were excised as described above and exposed to 30/30 min of global, no-flow ischemia and reperfusion under control conditions (n = 7) or in the presence of 64M 4-ClDzp (n = 5)

This process makes existing cells larger, but it does not create new muscle cells

Supplied as a 50mg lyophilised cake ready for room-temperature reconstitution