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Real-time monitoring of NADPH levels in living mammalian cells using fluorescence-enhancing protein bound to NADPHs

Hurley D, McCusker MP, Fanning S, Martins M

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In this experiment, the level of IGF-BP-3 decreased significantly and that of IGF-1 increased after the intervention, resulting in greatly increased molar ratio of IGF-1/IGF-BP-3, significantly higher than that of the other two groups

The expression of relative mRNA was normalized to that of the housekeeping gene 18S.Primer sequences used are provided in Supplementary Table 2