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Liu T, Zhang L, Joo D, Sun SC

Son HL, Park HR, Park YJ, Kim SW

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RNA interference To silence the expression of SMAD2 , SMAD3 , SMAD4 , or ALK-5 , GIC were transiently transfected by electroporation (Neon transfection system, Invitrogen) using siRNA pools (ON-TARGET plus human SMAD2 siRNA-SMART pool L-003561-00, ON-TARGET plus human SMAD3 siRNA-SMART pool L-020067-00-0020, ON-TARGET plus human SMAD4 siRNA- SMART pool L-003902-00 and ON-TARGET plus human ALK-5 siRNA- SMART pool L-003929-00 (Dharmacon, Lafayette, CO)

i , Conversion of ultrafiltered flavan-3,3,4-triol ( 4 ) into cyanidin ( 8 ) using 2 M purified Pt GSTF8 and different concentrations of GSH