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The manufacturer is explicit about this: frozen tirzepatide must be discarded even after thawing

[8] One standard drink is defined as 12 ounces of beer (5% alcohol), 5 ounces of wine (12% alcohol), or 1.5 ounces of distilled spirits (40% alcohol)

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The band densities of the proteins of interest were quantified by the NIH Image J and normalized by corresponding total actin bands