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Multi-photon and second harmonic generation microscopy The collagen gels were fixed with 4% paraformaldehyde for 20 minutes and washed in PBS with 0.1% saponin and 0.1% Tween 20 before being incubated with phalloidin conjugated with Alexa Fluor 594 for 1 h at room temp

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Equine -defensins Conclusions Competing interests Authors' contributions Acknowledgments 1

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