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[15,17] roGFPs are engineered to have two cysteine residues whose oxidation to form a disulfide induces structural changes that alter their fluorescence properties

We defined S to be a factor multiplying the V max values of GCL and GPX representing the activation of GCL and GPX, so S = 1 for males and S = 1.8 for females for average estradiol, since we make a case based on experimental and clinical results that the activation is by 80%

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Liposomal delivery and sublingual forms are designed to protect the molecule, allowing it to enter the bloodstream more effectively

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