and the generation of racemization impurities is greatly reduced, especially formation of [D-His1]-semaglutide is greatly reduced, formation of deletion peptides or polyaminopeptides in conventional one-by-one coupling, especially generation of [+Ser11]-semaglutide, [+Ala19]-semaglutide and [+Gly29]-semaglutide impurities, is effectively avoided, the purity of crude peptides is improved, downstream purification is facilitated, the material cost is greatly reduced, and the solid-phase synthesis method of the semaglutide is suitable for industrial production, and the total yield reaches 35%.
[6] The rapid pace leaves less time for metabolic adaptation, making proactive muscle and bone protection through myHMB and calcium supplementation critical rather than optional
20% Piperidine solution (50 ml of piperidine in 200 ml of DMF) was added to the above obtained reaction mixture at 25-30C and stirred for 40 min at the same temperature
98 g fully-protected peptides were obtained by recrystallization with a mixed solvent of methanol and dichloromethane
The transformed colonies were screened by colony PCR using T7 primers for the positive clones
