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After incubation in 2 mL of 0.05% trypsin-EDTA (25300054, Thermo Fisher Scientific) for 5 min at room temperature, cells were transferred into 8 mL of culture medium, centrifuged for 1 min at 200 g , resuspended in fresh medium and re-seeded into a new plate for continuous culture or seeded into a specific plate at the desired cell density, depending on the experimental setup

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The weight started coming on like never before, Bayandor said, adding that she was at the most shed ever weighed: 245

Oparaugo et al., 2023

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