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In contrast, the DOXHCl solution remained stable under both dilution conditions (Figure 3), indicating a good dilution stability in clinically relevant water matrices

Microwave-assisted ultrasound extraction is a hybrid method that combines microwave and ultrasonic treatments to enhance the yield of phenolic compounds, reduce extraction time, and minimize solvent usage compared to ultrasound-assisted extraction and microwave-assisted extraction alone (49)

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Therefore, we evaluated whether sulforaphane protected against rotenone neurotoxicity by modulating autophagy using the LC3-II biomarker (see Introduction)