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Commonly reported triggers include spicy foods, citrus fruits, tomatoes, chocolate, caffeinated drinks, alcohol, artificial sweeteners, overexertion, prolonged sitting, exercises that work the abdominal muscles (eg, sit-ups and crunches), riding a bicycle or horse, sexual activity, and wearing tight clothing

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Methods Animals and diets All animal experiments were performed according to procedures approved by the Northwestern University Institutional Animal Care and Use Committee, using male mice on a C57BL/6J background

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