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Epinephrine responsiveness is reduced in livers from trained mice

Where relevant, transfection was performed as for functional assays and surface labelling of SNAP-tagged GLP-1R was performed using 0.5 M of the indicated SNAP-Surface probe for 30 min at 37C before washing with HBSS

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A mechanistic link reduced active GLP-1 via microbiome/bile-acid changeshas now been demonstrated in humans