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VCAM-1, vascular cell adhesion molecule-1

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Lysates were clarified by centrifugation at 10,000 g at 4 C for 10 min, and the resulting supernatant was incubated with 1 ml of Glutathione Sepharose 4B beads (Cytiva, GE17- 0756-01) for 1 h at 4 C