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The samples were resuspended in 1 ChIP Buffer with PIC, incubated on ice for 10 min, and then sonicated using a Bandelin ultrasonicator (Sonics, United States) set to 300 W, 50 cycles (1 s each with a duty cycle of 50%)

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Studies in normal mice

Human pancreatic islets were isolated via collagenase digestion and purified from four non-diabetic human donors (Supplementary Table S1) as described previously 44