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Membranes were developed using 1:1 Amersham ECL western blotting reagent (GE Healthcare) to SuperSignal West Femto Chemiluminescent Substrate (Thermo Fisher Scientific) and detected by autoradiography

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This also explains why some lesions that appear less spectacular from a histopathologic standpoint (e.g., acral melanoma in situ, sometimes with very subtle histopathologic changes that are difficult to differentiate from nevus) progress rapidly to invasive melanoma if they are not correctly diagnosed on time