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cDNA was amplified in MicroAmp Optical 96-well reaction plates with SYBR Green PCR Master Mix (Applied Biosystems) on an Applied Biosystems Prism 7000 sequence detection system

For metabolite clustering analysis, average linkage hierarchical clustering was performed in the Statistical Analysis module of MetaboAnalyst 6.0 using Euclidian distance as a similarity metric

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The NRF2 target gene set ranges from ~30500 genes depending on publication (Kim et al, 2016