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The methodology for the HO scavenging assay can vary but generally involves preparing a solution of HO at a specific concentration, mixing it with the test sample, and measuring the absorbance at a defined wavelength (often around 230 nm) after a set incubation period (Kang et al., 2017

These results supported that compound 102 was a specific GPX4 activator, and increasing GPX4 activity would be an alternative way to suppress ferroptosis

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