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Enhanced Tim-3 expression in subpopulation of hepatic macrophages of mice with methionine and choline deficient diet indicated evidences, supporting the important role of Tim-3 in alleviating liver injury by regulating ROS production and proinflammatory cytokine secretion from macrophages [87]
To be used: Body
Western blotting Frozen cortex or hippocampal samples were homogenized in 50 mM TrisHCl (pH 7.5), 150 mM NaCl containing 1% Triton X-100, protease inhibitor cocktail and phosphatase inhibitor cocktail
That uncertainty matters when a symptom could be mild, progressive, or serious
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