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Cell Signaling Technology, Danvers, MA) was used and staining was performed on the Benchmark Ultra-automated Stainer (Ventana) using diaminobenzidine as the chromogen

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The exogenous testosterone may alleviate glycolipotoxicity-related mitochondrial dysfunction through the regulation of key enzymes involved in glucose and lipid metabolism, including adipose triglyceride lipase (ATGL), phosphoenolpyruvate carboxykinase (PEPCK), and proteins including glucose transport protein 4 (GLUT4) (129)

382,383 Daytime blue light therapy is sometimes added to chronotherapy to support daytime alertness and enhance melatonin function in the evening, and may improve measures of daytime functioning and sleep quality