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Melatonin restricts the viability and angiogenesis of vascular endothelial cells by suppressing HIF-1alpha/ROS/VEGF

Membranes were sequentially incubated with primary antibodies (anti-GLP-1 [Santa Cruz, sc-80603, 1:1,000], anti-MPC2 [Proteintech, 20049-1-AP, 1:2000], anti-GAPDH [Cell Signaling, D4C6R-97166, 1:5,000]) overnight at 4 C, followed by HRP-conjugated secondary antibodies (Santa Cruz, sc-2354, 1:5,000) for 1 h at RT

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Peptide therapy can help counteract the effects of aging, improving skin health, muscle mass, and overall vitality