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Subsequently, added 60 L of 50% acetonitrile, vortexed with shaking at 1700 rpm for 10 minutes at 8 C, centrifuged at 15,000 g for 20 minutes at 4 C, and aspirated the supernatant and stored at 80 C until analysis

Mice aged 1012 weeks, weighing 2528 g, were used for the experiments

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