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Tissues were permeabilized with PBS supplemented with 0.1% Tween-20 for 10 min, followed by a peroxidase block with 3% H 2 O 2 (Sigma-Aldrich, 216763) for 20 min

Additionally, acetyl-L-carnitine has been found to reverse age-related declines in carnitine palmitoyltransferase 1 activity in specific heart mitochondria, enhancing their ability to process fatty acids and maintain energy production.[19] Such supplementation may help sustain energy levels, muscle mass, and overall vitality, offering a potential strategy to counteract the effects of aging.[20] More research is needed to confirm whether or not such benefits would apply to humans

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2025;37:36176.e7

Early data suggest complementary effects when both agents are metabolically relevant, but robust clinical trials are ongoing