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however, following APAP treatment, LKO livers exhibited reduced JNK activation and less mitochondrial injury

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The cells were then centrifuged at 10,000 g for 2 min and incubated with 1 ml of 2 M HCl and 0.5% Triton X-100 (Sigma-Aldrich, 93443) for 30 min with gentle agitation

Qi X, Sun Y, Xiong S (2015) A single freeze-thawing cycle for highly efficient solubilization of inclusion body proteins and its refolding into bioactive form

doi:10.1186/1756-8722-4-10 Afrin LB, Butterfield JH, Raithel M, Molderings GJ