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Materials and methods Cell lines and cell culture Small cell lung cancer cell line NCI-H69 was purchased from the American Type Culture Collection (Rockville, MD, USA) and maintained in RPMI-1640 (Cellgro, Herndon, VA, USA) supplemented with 10% (v/v) fetal calf serum (FCS), L-glutamate, sodium pyruvate, and HEPES buffer as described previously (Ma et al, 2003a)