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Based on these estimates, a single, optimized standard addition mixture was custom prepared so that the additions approximated integer multiples of the endogenous concentrations, thereby maximizing method accuracy

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Oto najwaniejsze z nich: Signij po pokarmy bogate w siark Siarka to budulec cysteiny, jednego z trzech aminokwasw potrzebnych do produkcji glutationu

Cell-free supernatants were collected and stored at -20C for the future assays and granulomas were harvested by adding ice-cold sterile 1x PBS to wells, followed by gentle scraping to achieve maximum recovery of granuloma lysates and stored at -20C