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Ions for fragmentation were determined from an initial MS1 survey scan at 70,000 resolution (at m/z 200), followed by higher-energy collisional dissociation of the top 12 most abundant ions at a resolution of 17,500

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milliliters) because insulin dosage is measured in biological units. In standard U-100 insulin preparations, 1 mL = 100 units of insulin

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Oxidative Medicine and Cellular Longevity, 2012, Article 324832