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The observation that PARP inhibition partly rescues vitamin C-treated cells may suggest that the toxic effect of DHA uptake is not caused by ROS alone, since restoring NAD + levels and glycolysis with the PARP inhibitor may actually decrease PPP flux and NADPH production, and aggravate the redox stress

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2.11 Banding patterns of proteins using the SDS-PAGE technique Among the most effective methods for assessing mutagenicity is sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) caused by environmental pollutants, such as chemicals, and it also explained a genetic structure that was connected to the variety created by genotoxic effects of the genetic material [14, 15]