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They were then fixed in 4% paraformaldehyde (PFA) in phosphate-buffered saline (PBS) for 15 min at room temperature

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In both healthy and obese subjects, a high-protein meal increases plasma PYY levels significantly more than an isocaloric meal high in carbohydrate or fat, while PYY null mice are resistant to the satiating effects of protein (128)

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To lessen toxicity, mitigation techniques may include stronger restrictions, improved waste management, and biodegradable alternatives [118, 129]