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12, no obvious toxic reaction was identified in all groups, indicating the good biocompatibility of the hydrogels

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The mustard seed extract was testified for APAP-induced toxicity in a hepatocellular carcinoma cell line (HepG2) and was found to have protective effects in both post and pretreatment models with a 20 mM APAP dose (Parikh et al., 2015)

The serum was then centrifuged in the laboratory at 3500 rpm for 10 min to separate it from whole blood