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Based on preclinical (animal and cellular) data only, inhibition of NNMT by 5-Amino-1MQ is theorised to: Raise intracellular NAD+ levels Activate SIRT1 and other sirtuins (proteins linked to metabolic regulation) Reduce fat cell (adipocyte) size and proliferation Improve insulin sensitivity These effects have been demonstrated in mouse models (Neelakantan et al., 2019), but robust human clinical trial data are currently absent

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These polymorphisms are unrelated to cobalamin metabolism and the elevated MMA levels do not, by themselves, indicate a B12 deficiency (Molloy, 2016)

Vegans, pregnant women, and those with malabsorptive disorders could benefit from B vitamin supplements to prevent deficiencies

While effective for symptom control, NSAIDs carry risks of gastrointestinal ulceration and bleeding, cardiovascular events, renal impairment, and impaired bone and tendon healing